Hoechst33342+PFA - (Jun/23/2011 )
I want to use Hoechst33342+4%PFA to stain and fix my cells at the same time, 20min 37degree, and then wash one time. Is it possible?
I just want to aviod one washing step coz my cells is easily detached.
Should be fine I think- they did it like that here in this paper (http://www.ncbi.nlm.nih.gov/pmc/articles/PMC96081/)