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Hoechst33342+PFA - (Jun/23/2011 )

I want to use Hoechst33342+4%PFA to stain and fix my cells at the same time, 20min 37degree, and then wash one time. Is it possible?
I just want to aviod one washing step coz my cells is easily detached.


Should be fine I think- they did it like that here in this paper (