Analysis of a Chromium Release Assay - Prism or SPSS (May/31/2011 )
Hi,
I would like to analyze data from a chromium release assay (Effector:Target ratio on X-axis and lysis on Y-axis), but I am stuck on how to analyze the data (I have Prism and SPSS). I could of course analyze en E:T point, but I would like to analyze each line against another line.
Anyone who have had any experience with this?
Best Regards, Jonas
Usually people analyze simply by running t-test for each E:T ratio. If you wish to analyze whole curves against each other, then you should fit with some well-known curve (so you can interpret its parameters) and then compare the parameters. You can fit linear curves in prism and compare slope/intercept (prism outputs confidence intervals)
Hi,
I talked to a statistician and he suggested doing an Area Under the Curve analysis, so I think I will try that.
Thanks, Jonas
mike.sh on Sun Jun 5 23:47:30 2011 said:
Usually people analyze simply by running t-test for each E:T ratio. If you wish to analyze whole curves against each other, then you should fit with some well-known curve (so you can interpret its parameters) and then compare the parameters. You can fit linear curves in prism and compare slope/intercept (prism outputs confidence intervals)