Isolation of primary myoblasts - (May/22/2011 )

Hey,

looking for a protocol to isolate myoblasts from my Knockout and WT mice.
Has anybody here worked with primary muscle cells? What's your experience with these cells?

Thank you for you help.

Podge

In the meantime I have found this great protocol:
My link

Podge on Mon Jun 13 15:10:28 2011 said:

Yup, I got a couple of protocols for that... In my experience myoblasts are best isolated by first isolating satellite cells and then differentiating them into myocytes... Once in culture you will get a quite high level off diffentiation but it will never be completely free from undifferentiated cells and most likely also fibroblasts...