Protocol Online logo
Top : New Forum Archives (2009-): : MTT, Proliferation and Cytotoxicity Assay

MTT different from thymidine - (May/12/2011 )

Hi,
I did proliferation assay that showed decrease in thymidine intake but in the MTT I got no change
what does it means?
thanks
Michal

-michalcn-

michalcn on Thu May 12 19:50:16 2011 said:


Hi,
I did proliferation assay that showed decrease in thymidine intake but in the MTT I got no change
what does it means?
thanks
Michal

Thymidine incorporation reflects nucleic acid synthesis whilst MTT metabolic activity. Your cell may have stopped synthesizing NA but still be alive and metabolically active.

-klinmed-