Problem loading DNA / agarose gel - (Apr/21/2011 )
Dr_Watson on Fri Apr 22 13:19:05 2011 said:
thank you all for your replies and if you think of anything else, just let me know
have a happy easter, or Bonnes paques or whatever good weekend!!
You too, and bonne chance!
So latest news, I've made a new gel concentrating on the buffer. Which means, I used the same amount of DNA, same loading dye, not even bothering to centrifuge the samples this time. So as I said I focused on the buffer, adding at least 300mL of buffer on top of the gel and this time everything was ok. DNA stayed in the wells.
so just letting you know