smear in pcr +DNA polymerase mixture for long PCR - (Apr/15/2011 )
grvsomani on Mon Apr 18 04:14:36 2011 said:
tea-test on Sat Apr 16 16:06:13 2011 said:
try finnzyme's phusion enzyme. with fermentas i got only smear, phusion worked perfect for full length gene amplification, especially the hot start enzyme.
i have got long pcr taq enzyme from fermentas . i think it should work. give me ur opinion. what precautions should be taken for using it