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smear in pcr +DNA polymerase mixture for long PCR - (Apr/15/2011 )

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grvsomani on Mon Apr 18 04:14:36 2011 said:

tea-test on Sat Apr 16 16:06:13 2011 said:

try finnzyme's phusion enzyme. with fermentas i got only smear, phusion worked perfect for full length gene amplification, especially the hot start enzyme.


i have got long pcr taq enzyme from fermentas . i think it should work. give me ur opinion. what precautions should be taken for using it

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