Agarose Gel Disposal - (Apr/14/2011 )
Hi,
We just discarded in trash.
About EB, we prepared the gel without EB, then after running the gel Immersed it in EB for few min, then destain in dH2O..
-noyara-
pito on Mon Apr 18 13:30:34 2011 said:
lab rat on Sat Apr 16 16:33:35 2011 said:
Yep, I meant in the sunlight, since EB breaks down under UV. I was also told by an older tech that you could throw away only so much per day in the regular trash. Sometimes our gels would sit for a few days before discarding.
To answer Pito's comment, we're on a higher latitude, so we have summer sunlight from 5:30 am to 9 pm. Winter, we have sunlight at noon...sometimes.
Do you have some more information (paper?) on the details about the break down of EB under sunlight or uvlight?
I do not. I was told this by the people who trained me to work in the lab. I'll look, though.
I'll also point out that I said that we
-lab rat-
I guess normally you cannot escape the hazardous waste regulations of your institution...useful or not. We let the gels dry, to reduce he volume a lot and then they go to special plastics containers for hazardous waste. They are picked up by a company from time to time and I guess then incinerated, too...
-hobglobin-
well...no way to get rid of them totally 
but can't we reuse them again in less specific staff..!
-strawberry-