ChIP on sorted T lymphocytes - (Apr/07/2011 )
I'm just starting to standarize a protocol for Chip'in T lymphocytes not having good results so far. I was just wondering if anyone could help me put with any protocol using T lymphocytes using few cells (100.000).
There are micro-ChIP protocols out there for small cell numbers. just google it and a few papers should pop up, in fact, I just read a new one published in NAR I think. People use these protocols for ~10,000 cells