Protocol Online logo
Top : New Forum Archives (2009-): : Biochemistry

HPLC - Solvent peak (Apr/06/2011 )

I would like to know whether methanol gives any peak in C18 analytical column in HPLC.

-Dr. Mukherjee-

I presume you are thinking of Abs210 detection. In which case there is a small amount of absorbance from methanol. In addition there is the usual solvent front peak as a new solvent moves through the column.