difference between mouse and sheep antibody - sandwich ELISA (Mar/25/2011 )

Hi,

Do you think, that there is a difference between using mouse and sheep antibodies for the detection of staphylococcal extracelullar protein (enterotoxin G) - in cultivating media? Concretely, I have protocol which is optimized for sheep antibody...will it also work with mouse antibody?

Thank you for any advice or suggestion!

lucky2610 on Fri Mar 25 18:06:29 2011 said:

Hola, I think that if you use the recommended dilution for ELISA for the mouse ab you will have the same results.This is for detection as you ask, but if the ab was used for coating you have to use the same protein concentration that for sheep. I hope that you will have more clair answers afterwards. Buena suerte

protolder on Mon Mar 28 05:54:53 2011 said:

Hi,

thank you for your opinion! I hope that it will work;o) I only was affraid that something from staphylococcal growth medium could unspecifically bind to mouse antibody (protein A or G...but they are probably intracellular protein, aren't they?) and produce false positive signals...

I think you asked a question earlier about doing an elisa method for measuring this analyte. You indicated you had a method that uses sheep antibody and you want to know if you can use mouse. There is no guarantee that just swapping antibodies is going to give you a working assay not to mention the same performance. You can substitute one for the other and try same concentrations conditions etc...