Fat bands on 15% SDS-PAGE gel - (Mar/19/2011 )

i think that a component of your ripa is disrupting that part of your gel. try running the standards in a gel alone (don't add ripa to the standards, if you have been).

if you still see the fat bands then it could be that your sds has gone bad. try making fresh buffers with a different, newer, lot of sds.

if that doesn't solve the problem then try running a gradient gel. that should sharpen up the banding.