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how to prepare carbonate buffer - for elisa (Feb/23/2011 )

Hi, i wanted to prepare carbonate buffer for my ELISA. it's my 1st time :P

my question is, can we autoclave sodium bicarbonate and sodium carbonate?

from the protocol i saw, most of the people using filter sterilize method.

-badguy-

you should filter sterilize if you need it to be sterile.

but, why sterilize?

we make it fresh when we use it and don't sterilize.

-mdfenko-

mdfenko on Thu Feb 24 20:11:09 2011 said:


you should filter sterilize if you need it to be sterile.

but, why sterilize?

we make it fresh when we use it and don't sterilize.


can we autoclave it?





-badguy-

i wouldn't. carbonate buffer is not very stable.

-mdfenko-