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293T apoptosis - (Feb/07/2011 )

Hi,

Could anyone tell me a good protocol for the induction of apoptosis in HEK293T cells? Thank you a lot.

-CCWW-

Drugs - tamoxifen, paclitaxel, any of the DNA damage drugs. UV - find a cross linker (e.g. stratalinker) or other UV source with defined output and use that to titrate the amount needed to induce apoptosis in a given time frame.

-bob1-

Thank you bob1!!!


bob1 on Tue Feb 8 01:53:06 2011 said:


Drugs - tamoxifen, paclitaxel, any of the DNA damage drugs. UV - find a cross linker (e.g. stratalinker) or other UV source with defined output and use that to titrate the amount needed to induce apoptosis in a given time frame.

-CCWW-