Transgenic mouse model to measure Ca2+ in confocal - (Jan/12/2011 )
does anyone know a transgenic mouse model to measure Ca2+ levels in neuronal tissue by confocal microscopy (probably by FRET? any hint will help...
I suppose this is a culture then, and not a mouse on the table.
o.O
hmm, not sure if this will help...
http://www.springerlink.com/content/n64v8287752gr0k0/#section=98020&page=1&locus=5
..I don't think Ive seen much on FRETing neural tissues, you might want to check out other models such as alzheimers, maybe it will lead to a clever solution..
I'm not completely sure why you're looking at transgenic models for intracellular calcium measurements. Fura-2 is a standard ionophore for measuring calcium levels in cells in response to chemical stimuli. You might also look at Cameleon, a CamKII-GFP hybrid protein that can be transfected or transduced into cells to visualize calcium concentration.
that is the thing: If you have mutant mice stably expressing those calcium-senstive fluorescent proteins you can measure calcium in living mice...
gfischer on Thu Jan 13 18:24:13 2011 said:
I'm not completely sure why you're looking at transgenic models for intracellular calcium measurements. Fura-2 is a standard ionophore for measuring calcium levels in cells in response to chemical stimuli. You might also look at Cameleon, a CamKII-GFP hybrid protein that can be transfected or transduced into cells to visualize calcium concentration.