How to prevent self ligation - selfligation (Dec/19/2010 )
hi thanks... i will troubleshoot my work again and see how it goes...
-adrian kohsf-
bob1 on Mon Dec 20 21:27:37 2010 said:
If the vector is a problem - dephosphorylate it!
I agree with this, this is an easy step. I always dephosphorylate my vector before ligation.
-genesquirt-