Storage of Coated ELISA plates - Storage of Coated ELISA plates (Dec/17/2010 )
Has any one found a cheap and reliable way to store coated or coated/blocked ELISA plates, preferably in liquid form in the fridge.
We tried the dry and freeze method and it works but it seems to introduce a lot of variability especially around the plate edges. Also it is a pain and takes up -70 freezer space and they may get thrown around when other people get their stuff out.
There are a number of commercial products out there that make a lot of claims. Has anyone tried any of them? Applichem and Candor have products but they cost anywhere from 5-20$ per plate. They also claim to increase signal to noise and reduce plate to plate variability. Maybe they will make me breakfast too ..
Even with these products I would probably have to do stability studies anyway.
What are peoples thoughts on how long a properly sealed antibody coated ELISA plate can last in the fridge without significant loss of binding? It may be Ab specific, but I have tested week old plates and there didn't seem to be any obvious signal drop, but that is all I have had the nerve and time to do.
1. You can leave your blocking solution in the plate and seal the plate and store in humid chamber to avoid evaporation. Plates will probably last a couple of weeks or longer.
2. Better long term approach. After you block your plates, decant and blot...fill the wells with a PBS/sucrose solution. Leave for 30 min - 2 hr then decant...do not blot....invert and dry overnight at RT. The sucrose will form a layer over the abs/ag/blocking protein and protect the proteins. Then place the plates in a plastic bag with dessicant.
there are other 'blocking/storage' buffers from several other manufacturers but the sugar coating worked for us for a commercial product and was very cheap!