lipid permeability - (Dec/02/2010 )

Hi All ,
I want to stain helminth eggs but the problem is it has a lipid layer which is highly impermeable. Does any one have any idea about improving the permeability of lipid layer ( espeacially ascaroside layer)? like treating with xylene, or sonication or electoporation?

What have you tried so far? I would start with detergents - SDS, triton X-100, NP-40, etc.

bob1 on Thu Dec 2 22:02:20 2010 said:

Thankyou very much for the reply. I have not yet tried any but planning to pretreat the eggs with some enzymes.But will try some of the detergents you mentioned.