How to convert ng/ul to ug/ul - (Nov/18/2010 )
I know it seems like such an easy concept, but if someone could give a helping hand in helping me understand the concept, I would greatly appreciate it!
So from this website, I gather that if I have a starting concentration of 500 ng/ul and my desired concentration is 0.1 ug/ul with a final volume of 100 ul, the amout of sample to dilution water would be 20 mL solution and 80 mL water.
Could someone explain this to me?
Thank you so much for your time!
1 ng/ul = 0.001 ug/ul
Use V1 x C1 = V2 x C2 (where V = volume and C= concentration) Keep the units the same (i.e. if using ul, keep it all in ul).
0.1 ug/ml is 100 ng/ml. 500 ng/ml is 5 x 100 ng/ml meaning you have to dilute 5-fold => 20 ul + 80 ul
let just show you an easy way to convert between units :
ng to ug ...
convert the nanogram to grams, then the grams to micrograms ...
for example 5 ng = ?? ug
5 ng x 1 g = 5 x 10 ^ -9 g
10 ^ 9 ng
(5 x 10 ^ -9 )g x (10 ^ 6) ug = 5 x 10 ^ -3 ug
so 5 ng = 0.005 ug
without the need to memorize anything as most students do ...
hope i have simplified it !