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mouse brain fixation - (Nov/14/2010 )


I would like to know for how long you could fix a mouse brain (P5 -P15) if you don't use the perfusion method, but instead incubation in 4% PFA. Do you need days or some hours are enough? I want to cut cryosections and use them for in situ hybridization.

Thanks a lot in advance!


24 hrs in PFA should be fine for mouse brain even if not perfused.