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Top : New Forum Archives (2009-): : ELISA and Immunoassay

Pathscan sandwich ELISA - (Nov/02/2010 )

I have bought a Pathscan sandwich elisa kit for acetylated histoneH3. they havent given any standards or controls. how to i interpret my results? how do i quantify? do i have to add cell lysate in such a way that it has equal amount of total protein? (the kit just says 100microlitres of cell lysate).


you can find the protocol at the cell signaling technologies website.