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Alternative software for Geneious - expired software... (Oct/12/2010 )

Dear All,
I am looking for software, preferably freeware for my bioinformatics work.
Briefly, my work begin after DNA sequencing where I align the forward and reverse sequenced together based on the chromatogram and perform manual alignment when there is any ambiguous. Finally I will export the consensus (based on the quality of chromatogram) for further work.

Geneious works pretty easy for me to work but however my trial version is getting expired. I was wondering if there are any program which could work the same way.
I tried CLC sequence viewer but however it doesn't shows the quality of the chromatogram. I heard about NTI vector but is not free.

Any suggestions?

Thanks.

Adrian

-adrian kohsf-

you can try codoncode aligner.

-mdfenko-

Thanks mdfenko, now I'm downloading it and try out...

Anyone uses any other "free" program?

-adrian kohsf-

CodonCode Aligner does exactly what you need to do. Unfortunately it is not free. If you familiar with Unix, there is a software combiner, Phred, Phrap, and Consed. They do exactly what CondonCode Aligner does in window system.

-subtelo-

there's also the staden package.

-mdfenko-

Hmn... staden package is interesting, but then no choice but I have to learn and install unix system...

Anyone knows ss there any shortcut or tutorial to make it (staden etc) usable in windows?

Thanks.

-adrian kohsf-

the latest windows installer version of the staden package is 1.7.0.

the author will eventually prepare an windows installer for the latest version.

-mdfenko-

Hi mdfenko.
Thanks for your reply.
I just got the link and is now downloading the software.

^_^ Cheers
Adrian

-adrian kohsf-