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please suggest methods to detect apoptosis - (Oct/01/2010 )

Hello,
Please suggest me methods to detect apoptosis in tumor tissue slides as well as samples samples.
I did Tunnel staining but I am unable to find tunnel positive cells due to high background.
I will appreciate the help.
thank you.

-deep-

hello :)

kindly read the attached article, hope you will find it beneficial.

Attached File

Best Wishes

-nightingale-

check for the cleaved form of caspase 3!

-moljul-

nightingale on Mon Oct 4 20:15:40 2010 said:


hello :)

kindly read the attached article, hope you will find it beneficial.



Best Wishes


Thank you for tthe suggestion..
Deep

-deep-

If epithelial cells, you can also try measuring cytokeratin 18 (ELISAs are available for few different methods)

-Wiggum-