Anticoagulants assay - (Sep/21/2010 )

Hi,
We would like to compare a protein with other anticoagulants to check its anticoagulant function.
Does anybody else has protocol, know whaat method should I use or where can find a good protocol?


Any help would be greatly appreciated.


Xfu

Protein coagulate when the internal material will stick together and whole protein structure will shrink....so regarding protocol i think you should suppose to study the different internal material of the proteins and then accordingly add the anti-coagulant as we do in testing blood groups.
For Further information you can study form NCBI.

You can also check out antibioticos clasificacion of wiziq to add on to your knowledge.It will be beneficial to you in future.

Regards
Rupam

Thank you very much for your help.

Rupam on Sat Sep 25 12:45:42 2010 said:

Purchase Innovin from Siemens which will start coagulation in 15 seconds or less. You can add your 'anticoagulants' to blood or plasma and determine at what concentrations the coagulation time is extended. All you need is sample, Innovin, stop watch and microscope slides...pretty simple.

Hi,

i am the technical service with Cell Counter . What is the recommendation for anticoagulant ratio between K3-EDTA or K2EDTA or EDTA and 100 micro liter blood ( Child samples & Neonate Samples ).

Regards/ Madhavan