dose incubation in 16 C increase the ligation efficiebcy? - (Sep/11/2010 )
ElHo on Wed Sep 15 12:54:47 2010 said:
Qiaquick gel extraction kit (Qiagen), but I´m also losing a lot with that one. But is the roche kit also suitable for gel extraction? I always thought it`s only used for PCR cleanup. Do you have to gel purify your PCR? Otherwise you could try to just purify your PCR product without gel extraction. Then you certainly will end up with more material to ligate.
yea, the roche kit is designed for both pcr reaction purification and gel purification. I kinda have to extract the fragment form gel, 'cos there are unspecific pcr amplified fragments as well.