uv vis spectrophotometer - (Aug/23/2010 )
what is the purposes using uv vis spectrophotometer?
it is necessary to use this equipment after gel filtration??
there are a lot of uses for a uv/vis spectrophotometer.
what i think you are asking is if you need it to find and/or quantify the protein in your fractions and the answer to that is: maybe. it depends on what you already know and what you need to find out and where you are going from there.
in general, for instance, to monitor enzyme assays based on the NAD/NADH system; you should check at 260 nm and 340 nm
for maxima of absorption;
as mdfenko already answered to monitor amounts of protein,e.g. integrated in a HPLC/FPLC system...