How to accurately pipet blood? - (Aug/07/2010 )

I use two methods for accurately pipet blood.
With a tip
First pipette the blood very slowly. When taking blood in let the piston come up slowly and wait for 20-30 seconds so you're sure the blood is in.
Then slowly push the piston down and watch very carefully if all the blood from the wall of the tip is coming down, if not you're pushing to fast. It,s a very slow proces it can take minutes.
(With a glass pipet it works the same)

Much faster is using a positive displacement pipette as mentioned before.

Weight is not a solution because the specific weight of blood is not known therfor you can't calculate the volume.

Gerard on Sun Aug 22 09:14:58 2010 said:

You can use the pipette of 1000 microlitre and pipette out 1000 ml blood for rest 500 ml you can set the 1000 microlitre pipette to 500 and pipette out the rest blood.It will give the accurate blood amount.

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Regards
Rupam

The use of reverse pipetting helps. Just pushed right to the 2nd stop of the pipette and draw the fluid in excess, dispense only to the 1st stop. This will accurately dispense the amount without any retention in the pipette tip.