standard curve method for Q-PCR - (Aug/05/2010 )
Hi everyone I am a bit confused about some of the methods used to analyse Q-PCR data. I have read a bit about primer efficiencies, do you need the primer efficiencies of your target and reference genes to be similar if you are quantifying using the standard curve method?
As far as I know, no. Only if you use deltadeltaCT. And even then itīs not an absolute prerequisite as you can use the method developped by Pfaffl.
Is the standard curve method as good as the CT method?
Quantification experiments by running standard curves in parallel are somewhat easier to design and fewer prerequisites must be met. But you also have higher material consumption.