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standard curve method for Q-PCR - (Aug/05/2010 )

Hi everyone I am a bit confused about some of the methods used to analyse Q-PCR data. I have read a bit about primer efficiencies, do you need the primer efficiencies of your target and reference genes to be similar if you are quantifying using the standard curve method?

-cellculturenovis-

As far as I know, no. Only if you use deltadeltaCT. And even then itīs not an absolute prerequisite as you can use the method developped by Pfaffl.

-ElHo-

Is the standard curve method as good as the CT method?

-cellculturenovis-

Quantification experiments by running standard curves in parallel are somewhat easier to design and fewer prerequisites must be met. But you also have higher material consumption.

-ElHo-