prolonging survival of live brain slices - (Aug/04/2010 )

I have a classical protocol but are there any rarely-documented helps to prolong survival and conductivity of brain slices?

We add 400 uM ascorbate to the aCSF.

see:
Use of Ascorbate in the Preparation and Maintenance of Brain Slices

Rice 1999

this is what I was looking for, thank you very much...