Serial Dilution - (Jul/20/2010 )
Sorry just a quickie - I am having a brain freeze and hope you can help!
At the moment our 6 standards are made up of 3 solutions, but the 6 are made up seperately.
sol A sol B sol C
1 1 ml 5 ml 5 ml
2 1 ml 6 ml 4 ml
3 1 ml 7 ml 3 ml
4 1 ml 8 ml 2 ml
5 1 ml 9 ml 1 ml
6 1 ml 10ml 0 ml
I'm sure this can be serially done but just can't figure it out today!!
I do not understand your question.
And how do you mean a serial dilution?
Solution A for example is always 1ml per 11ml total... there is nothing you can "dilute" ..
Also the other solutions: you cant dilute it...
you have to make it seperatly.
agree with pito, you are not dealing with a serial dilution here, but with different mixtures of the three solutions.
You will have to make each standard on its onwn; you have different concentrations of every component in each of your standards;
The amount of solution A stayes fixed, whereas the concentrations of sol b and c are ascending/descending indirectly proportional. the proportion of the three solutions is different and not symetric for all 6 mixtures.