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Any additional system for Hypoxia??? - (Jul/14/2010 )

Hello,
I'm a newbie in the field. Does somebody knows whether there is a simple system to culture my cell in hypoxia? I heared something about a special bag that can be kept in a classic incubator.

Thanks a lot for your help

-netdavid-

netdavid on Jul 14 2010, 04:40 PM said:

Hello,
I'm a newbie in the field. Does somebody knows whether there is a simple system to culture my cell in hypoxia? I heared something about a special bag that can be kept in a classic incubator.

Thanks a lot for your help


I dont know about those bags. I use a special incubator. But you can try chemical hypoxia

-laurequillo-

netdavid on Jul 14 2010, 04:40 PM said:

Hello,
I'm a newbie in the field. Does somebody knows whether there is a simple system to culture my cell in hypoxia? I heared something about a special bag that can be kept in a classic incubator.

Thanks a lot for your help

In the past I have used "modular incubation chambers" (see attached). We gassed these from pre-mixed gas cylinders.

I would imagine you could make a cheap system yourself using a sandwich box, tubing and a little epoxy glue.

Hope this helps
Attached File

-klinmed-

Thank you very much Laurequillo and Klinmed for your advices.

-netdavid-