No SDS in gels - (Jun/25/2010 )
I've never seen this kind of crackling as in the picture thus i'm guessing.
I,m not sure but looking at the picture i have the feeling that the problem originates from the gel casting or the imager. I don't think the blotting or the probing caused this problem.
-Gerard-
mdfenko on Jul 1 2010, 06:27 AM said:
why do you change blocking agent between antibodies? with what do you block the membrane?
you may want to consider adding normal sheep serum to the block and antibody solutions.
you may want to consider adding normal sheep serum to the block and antibody solutions.
I use BSA with my primary Ab because we re-use our primary antibodies after probing. Sodium Azide is added to it to preserve it for future use.
-Leon_K-
Gerard on Jul 2 2010, 09:59 AM said:
I've never seen this kind of crackling as in the picture thus i'm guessing.
I,m not sure but looking at the picture i have the feeling that the problem originates from the gel casting or the imager. I don't think the blotting or the probing caused this problem.
I,m not sure but looking at the picture i have the feeling that the problem originates from the gel casting or the imager. I don't think the blotting or the probing caused this problem.
You're right, after help from a colleague, it seems that i've used the wrong settings to image the blot. Also, i used regular ECL which gave a very weak signal. After using Super ECL, the bands were much clearer.
-Leon_K-