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Apoptosis analysis - (May/21/2010 )

Hi!

I am planning to perform RNAi and want to check tumor suppression. Is there any method other than flow cytometry i can use to measure apoptosis. I checked a few immunofluorescence and ELISA bases assays. Are these useful? Why is flow cytometry so comonly used?

-srm mrs-

srm mrs on May 22 2010, 05:47 AM said:

Hi!

I am planning to perform RNAi and want to check tumor suppression. Is there any method other than flow cytometry i can use to measure apoptosis. I checked a few immunofluorescence and ELISA bases assays. Are these useful? Why is flow cytometry so comonly used?


with FACs you can measure how many cells are at which cell cycle phase or cell cycle arrest, respectively;

other methods beside ELISA and FACS are light or fluorescent microscopy, immunoblot, DNA fragmentation

-Inmost sun-

Check this paper: http://www.ncbi.nlm.nih.gov/pubmed/19373242

-cardosopedro-

There are methods to measure apoptosis specifically by measuring cytokeratin 18 fragments with antibodies or ELISAs

-Wiggum-