Destaining a coomassie sttained gel - (May/13/2010 )
Type of concentration (ie. v/v, w/v or w/w) is not stated in the article and I assumed (for simplicity) that it is v/v.
I need 12.5% which is 125 mL of "pure acid" per 1000 mL
85% acid is 80 mL of "pure acid" per 100 mL
125 - x
80 - 100
(125*100)/80 = approx. 147
yeah i assumed that this is what you would have done!!!
(typo - there should be 85 not 80 in ratio calculation)
K.B. on May 19 2010, 02:22 PM said:
actually i will be doing a silver coomassie and a colloidal direct comparision in a day or two.. will let u know of the results!!!
See also Dyballa N, Metzger S. 2009. Fast and sensitive colloidal coomassie G-250 staining for proteins in polyacrylamide gels. J Vis Exp. Aug 3;(30):1431. (PMID: 19684561, here) and Pink M, Verma N, Rettenmeier AW, Schmitz-Spanke S. 2010. CBB staining protocol with higher sensitivity and mass spectrometric compatibility. Electrophoresis. Jan;31(4):593-8. (PMID: 20162584, here).