Protocol Online logo
Top : New Forum Archives (2009-): : Cell Biology

What is the pricinple behind 1.8g NaHCO3 per litter when CO2 is 5%? - (May/07/2010 )

When the partial pressure of CO2 is 5%,you should add 1.8g NaHCO3 to 1L DMEM (high glucose GIBCO),
when it's 10%,you should add 3.7g NaHCO3.
then what is the pricinple/theory/formula behind this?
How to convert?
I added 3.7g NaHCO3 per litter when CO2 is 5%,what is the harm?
Does it have anything to do with the relatively fast change of the color to yellow?

-AllenChiu-

AllenChiu on May 7 2010, 01:09 PM said:

When the partial pressure of CO2 is 5%,you should add 1.8g NaHCO3 to 1L DMEM (high glucose GIBCO),
when it's 10%,you should add 3.7g NaHCO3.
then what is the pricinple/theory/formula behind this?
How to convert?
I added 3.7g NaHCO3 per litter when CO2 is 5%,what is the harm?
Does it have anything to do with the relatively fast change of the color to yellow?


NaHCO3 + H2O = Na+ + H2CO3 + OH- = CO2 + H2O + Na+ + OH-

or

NaHCO3 + 2H2O +CO2 = Na+ + 2H2CO3 +OH- = 2CO2 + 2H2O +Na+ + OH-

NaHCO3 and CO2 will be in balance, regard the K for NaHCO3, and K for H2CO3; to calculate the correct percentage of CO2 you also need the incubator volume

-Inmost sun-

Inmost sun on May 10 2010, 04:54 AM said:

AllenChiu on May 7 2010, 01:09 PM said:

When the partial pressure of CO2 is 5%,you should add 1.8g NaHCO3 to 1L DMEM (high glucose GIBCO),
when it's 10%,you should add 3.7g NaHCO3.
then what is the pricinple/theory/formula behind this?
How to convert?
I added 3.7g NaHCO3 per litter when CO2 is 5%,what is the harm?
Does it have anything to do with the relatively fast change of the color to yellow?


NaHCO3 + H2O = Na+ + H2CO3 + OH- = CO2 + H2O + Na+ + OH-

or

NaHCO3 + 2H2O +CO2 = Na+ + 2H2CO3 +OH- = 2CO2 + 2H2O +Na+ + OH-

NaHCO3 and CO2 will be in balance, regard the K for NaHCO3, and K for H2CO3; to calculate the correct percentage of CO2 you also need the incubator volume

great,Thank you!

-AllenChiu-

Inmost sun on May 10 2010, 04:54 AM said:

AllenChiu on May 7 2010, 01:09 PM said:

When the partial pressure of CO2 is 5%,you should add 1.8g NaHCO3 to 1L DMEM (high glucose GIBCO),
when it's 10%,you should add 3.7g NaHCO3.
then what is the pricinple/theory/formula behind this?
How to convert?
I added 3.7g NaHCO3 per litter when CO2 is 5%,what is the harm?
Does it have anything to do with the relatively fast change of the color to yellow?


NaHCO3 + H2O = Na+ + H2CO3 + OH- = CO2 + H2O + Na+ + OH-

or

NaHCO3 + 2H2O +CO2 = Na+ + 2H2CO3 +OH- = 2CO2 + 2H2O +Na+ + OH-

NaHCO3 and CO2 will be in balance, regard the K for NaHCO3, and K for H2CO3; to calculate the correct percentage of CO2 you also need the incubator volume


But temperature (and presumable pressure) should also be considered, as it influences the balance too a lot...i.e. the specification should be completed with a fixed temperature such as 20C, or STP.

-hobglobin-