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purity is so bad after elution from GST column - (May/03/2010 )

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Another Jake on May 7 2010, 10:54 PM said:

NP40 (Igepal) is just another non-ionic detergent. If you've got triton already, you don't need it.

Also, when you go to elute from your nickle column, take a series of elution fractions. I've often seen multiple bands disappear in the later fractions. Also some of the bands might very well disappear after the subsequent GST protocol.
Good luck.

Thanks a lot!

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