PBS for ELISA - (Apr/25/2010 )

hi,

Is there a difference between PBS-/- and PBS +Mg+Ca when performing an elisa assay?

Thanks,

Avivit

Avivit on Apr 25 2010, 12:30 PM said:

You could use either, but it is normal to use PBS without Ca/Mg. One exception is if your antibody is dependent on Ca for binding (eg the anti-FLAG monoclonal M1).

Hope this helps.

Thanks that what we thought but weren't sure...

or it is needed for the enzyme to be active.