Protocol Online logo
Top : New Forum Archives (2009-): : General Biology Discussion

How to Improve EC50? EC50= Half maximal effective concentration - Please please help! (Apr/18/2010 )

We did an experiment for the MEASUREMENT OF AGONIST POTENCY which investigated the responses of a section of guinea-pig ileum (mounted in an organ bath) to acetylcholine and histamine.

How can we improve/obtain more reliable values of our EC50 value, given that we had time?? How


Any clues would be great. Thanks.

-jamescorden-

jamescorden on Apr 18 2010, 11:55 AM said:

We did an experiment for the MEASUREMENT OF AGONIST POTENCY which investigated the responses of a section of guinea-pig ileum (mounted in an organ bath) to acetylcholine and histamine.

How can we improve/obtain more reliable values of our EC50 value, given that we had time?? How


Any clues would be great. Thanks.



you have to re-done the experiment for several times, at least n = 3-times independently form each other which means using fresh preparations form other guinea pigs;

which effect of receptor stimulation are you measuring?

-Inmost sun-