Using Mitomycin in Wound Healing Assay - Protocol suggestions please... (Apr/14/2010 )
Hi all,
I would like to add 25ug/ml of mitomycin to prevent cell proliferation inorder to study the migratory rate of the cells in wound healing assay. I was wondering if I can add the above mentioned concentration of mitomycin for 25 mins at 37 deg C, and then remove media (containing mitomycin) and replace with normal media and continue with wound healing assay (for 6, 24 & 48hrs).
Can you please tell me if this is ok... thanks a ton!!!
-molecular_medicine-
molecular_medicine on Apr 14 2010, 04:13 PM said:
Hi all,
I would like to add 25ug/ml of mitomycin to prevent cell proliferation inorder to study the migratory rate of the cells in wound healing assay. I was wondering if I can add the above mentioned concentration of mitomycin for 25 mins at 37 deg C, and then remove media (containing mitomycin) and replace with normal media and continue with wound healing assay (for 6, 24 & 48hrs).
Can you please tell me if this is ok... thanks a ton!!!
I would like to add 25ug/ml of mitomycin to prevent cell proliferation inorder to study the migratory rate of the cells in wound healing assay. I was wondering if I can add the above mentioned concentration of mitomycin for 25 mins at 37 deg C, and then remove media (containing mitomycin) and replace with normal media and continue with wound healing assay (for 6, 24 & 48hrs).
Can you please tell me if this is ok... thanks a ton!!!
it should work; MMC will induce apoptosis
-Inmost sun-
Cheers, Ta...
Wondering if the cells will still migrate after MMC... Will it induce apoptosis even after removing it off from the media????
-molecular_medicine-
molecular_medicine on Apr 15 2010, 09:41 AM said:
Cheers, Ta...
Wondering if the cells will still migrate after MMC... Will it induce apoptosis even after removing it off from the media????
Wondering if the cells will still migrate after MMC... Will it induce apoptosis even after removing it off from the media????
what´s the mechanism? MMC is intercalated between the two DNA strands and, therefore, blocks replication or transcripton; this blocking induces apoptosis which is the sought effect in antitumor therapy; you wash the cells but a portion of MMC is already intercalated; at the late stage of apoptosis, cells will not migrate
-Inmost sun-