BSL1 waste sterilization - (Mar/24/2010 )
Can the BSL1 waste and daily used consumables be autoclaved in the same autoclave machine? If i do autoclave waste in that autoclave which is used to autoclave daily consumables, would it be okay. I mean is there any chances of contaminations to the consumables if we do autoclave BSL1 waste in the same autoclave machine?
You should not have problems usually the same autoclave is use for waste and the non waste. Use a long cycle for waste (45 min-1 h should be more than enough. The problem with waste autoclaving is the odor but there are some products that neutralize it.
merlav on Mar 24 2010, 12:39 PM said:
Thank you for your reply. Actually i usually use the cycle of 15 min at 121C for doing autoclave of tip boxes, tubes etc and waste as well. But i do it separately. Some people have objection that it is not recommended to use the same autoclave for waste. I think if we are dealing with BSL1 or even 2 grade waste then we can use the same autoclave for both. In case of L3 and L4 or medical waste we may think to have a separate autoclave.
autoclaving media and things that can't stand too much time at high temp could be at 121C 15 min or less, but plastic, crystalware can stand a little bit longer time. remember that time should be count when reach the temp/pressure not when start. Usually the waste is larger than plasticware so it will need more time to the vapor to reach and sterilized all and that's why it needs more time. don't close too tight the bag to let the vapor inside it, put the bag inside a tray to collect any liquid and sterilized for at least 45 min. I prefer 1 hour. Check regularly the function of the autoclave with thermophylus test (not only because the autoclave tape change color). if doing this you could use the same autoclave for all.
merlav on Mar 25 2010, 05:57 AM said: