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What does "seeding" mean? - (Jan/31/2010 )

Beginner to cell culture - please help! What does "seeding" mean?

-prof. moriarty-

Seeding simply means to spread a defined amount (volume or cell number) of a cell suspension into a new flask or onto a plate etc.
When you work with adherent cell cultures you have to trypsinize them first to get a cell suspension.
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