5-azacytidine concnetration - calculation (Jan/19/2010 )
Coul someone double check my concentrations:
I have a vial of 5-azacytidine from Sigma (catalogue no.: A 1287). The vial says 50X concentration and have to add 10ml medium to give 500uM. The working concentration in 10um.
If I need a working concentration of 10um in a total volume of 2ml:
40ul of 50X stock plus 1960ul of medium ?