Bleh, screwedup. How long before FBS goes bad - (Jan/05/2010 )
Yesterday afternoon, I pulled some FBS out of our -80 and put foil around it (to protect it from light while it thawed). I had planned on aliquotting it after it had thawed out for a couple of hours at room temp. Of course, I then forgot about it and just found it this morning. It doesn't look turbid, so do you think it's still good? It's been filtered 0.1 uM so I think it is sterile inside, and I think the foil would help it from degrading. What do you peeps think...?
If it's sterile, use a bit of it for culture and see if the cells are OK. I have forgotten unopened, sterile FBS in the 56C water bath for several hours and it was still fine.
If it's not sterile, then toss it.
It should be fine, but there might be a loss of some of the growth factors. I usually thaw overnight at 4 degrees C.