Transwell co-culture and thymidine proliferation assay - (Dec/28/2009 )
I have a problem. Both my cells are adherent cell, BV2 and MSC.
I have to determine the proliferation of BV2 after co-culture with MSC.
However, because both cells are adherent cells, i need to separate both cells with transwell.
Transwell only can fits into 6 well, 12 well and 24 well plate.
Meanwhile, proliferation assay must be prepared in 96 well plate!
1. trypsinize the cell and transfer from 12 well plate to 96 well plate?
2. is there anyhow i should do?
Why must the proliferation assay be done in a 96-well plate? seems that you could scale-up
because the machine only can read 96 well plate