Info about 3-Amino-1,2,4-triazole (3-AT) - (Dec/22/2009 )
I'm making up plates with 3-Amino-1,2,4-triazole, aka 3-AT (super tricky and time consuming plates ). I'm getting some unexpected results (not necessarily bad results, but weird) and I want to rule out the possibility that I'm mistreating the 3-AT.
Does anyone out there use it regularly?!
I've checked the literature. I've searched google. I've spoken with tech support at Sigma. I can't find any information regarding the typical storage conditions of 3-AT. I've got it at 1 M in water, filter sterilized and stored at -20. Is this pretty standard? How long is it good stored at -20? Should I be making small aliquots to avoid freeze-thawing? I thaw it in a room temperature water bath. Should I be making it up fresh? For the plates I'm making, I add the 3-AT to a solution of non-autoclavable reagents, filter sterilize, and bring it up to about 50 C. I then add that solution to an autoclaved agar solution that's at about 50-60 C. Is this OK? Is 3-AT stable at 50 C? For how long?
Any info would be GREATLY appreciated!!
In an answer to my own question, just in case anyone else is wondering about the stability of 3-AT.
Santa Cruz Biotechnology says:
3-AT is soluble in hot water up to 50 mg/mL (which is about 0.59 M). Interesting, because I was able to get it into solution at 1 M with only a little heat.
They also recommend making small aliquots and freezing them at -20. They say it's stable up to 3 months at -20.
Sigma gave me this:
The product is soluble in water, methanol, ethanol and chloroform, and sparingly soluble in ethyl acetate. It is insoluble in ether and acetone. Aqueous solutions are neutral.
I also found a reference to unpublished data amongst some herbicide info. It says the following:
The stability of amitrole (3-AT) was studied in buffer solutions at 90C at pH 4,7, and 9 and a concentration of about 10 mg/L. Samples were taken 0, 19, 94 and 114 days after application. No degradation was observed, indicating that the half-life of amitrole in water at 20 C would be more than one year.