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Inhibitor Concentrations - (Dec/07/2009 )

Hello, I am testing out an inhibitor in cell culture. How many different concentrations of this inhibitor should I use?
Also, what are the statistics involved in determining if an inhibitor is effective in a dose dependent manner?

-prof. moriarty-

you can find some of the information at these webpages:

how much should i use?

and here:

download the inhibitor sourcebook from here
look down on the page at "product guides available by download only"

these are pages at the emd (calbiochem) website.

-mdfenko-

Depending on the IC50 of the inhibitor. I used to start with 1uM and go all the way down to 10^-12uM. PRISM is really good software to build the dose response curves and anything related with pharmacological assays.

-medchemgirl-