Inflammation: Amyloid beta 25-35 vs. 1-40? - differences ab 25-35 and 1-40 for cytokine production (Nov/22/2009 )

Hi, I'm doing a science fair project involving effect of amyloid beta on tnf-a production in macrophages, and the inhibitory effects of various compounds. I know that ab 25-35 is a lot cheaper, and takes way less time to fibrillize, so there's definitely incentive to use it.

But the focus of my project is the "various inhibitors" part, and it would be useless if amyloid beta didn't induce tnf-a production. I've seen many studies showing that 25-35 induces tnf-a, but i've also seen a few showing that it didn't have any effect by itself, only with a proven macrophage activator like LPS. There's quite a few more studies showing that 1-40 is an inflammation inducer.

Do you any of you have experience with this? A company has offered to donate several materials including amyloid beta 25-35, but I don't know if they'd still be willing to donate if I changed it to ab 1-40 (which is a good $300-400 more expensive)

Thanks for your help.

p.s. sorry about my screenname, i'm using someone else's account - i don't want you to get the idea that i think myself a "guru" when i'm only doing a science fair project

~guru on Nov 22 2009, 02:31 PM said:

Hi, I'm doing a science fair project involving effect of amyloid beta on tnf-a production in macrophages, and the inhibitory effects of various compounds. I know that ab 25-35 is a lot cheaper, and takes way less time to fibrillize, so there's definitely incentive to use it.

But the focus of my project is the "various inhibitors" part, and it would be useless if amyloid beta didn't induce tnf-a production. I've seen many studies showing that 25-35 induces tnf-a, but i've also seen a few showing that it didn't have any effect by itself, only with a proven macrophage activator like LPS. There's quite a few more studies showing that 1-40 is an inflammation inducer.

Do you any of you have experience with this? A company has offered to donate several materials including amyloid beta 25-35, but I don't know if they'd still be willing to donate if I changed it to ab 1-40 (which is a good $300-400 more expensive)

Thanks for your help.

p.s. sorry about my screenname, i'm using someone else's account - i don't want you to get the idea that i think myself a "guru" when i'm only doing a science fair project

forrestlin on Apr 27 2010, 03:45 AM said:

~guru on Nov 22 2009, 02:31 PM said:

Hi, I'm doing a science fair project involving effect of amyloid beta on tnf-a production in macrophages, and the inhibitory effects of various compounds. I know that ab 25-35 is a lot cheaper, and takes way less time to fibrillize, so there's definitely incentive to use it.

But the focus of my project is the "various inhibitors" part, and it would be useless if amyloid beta didn't induce tnf-a production. I've seen many studies showing that 25-35 induces tnf-a, but i've also seen a few showing that it didn't have any effect by itself, only with a proven macrophage activator like LPS. There's quite a few more studies showing that 1-40 is an inflammation inducer.

Do you any of you have experience with this? A company has offered to donate several materials including amyloid beta 25-35, but I don't know if they'd still be willing to donate if I changed it to ab 1-40 (which is a good $300-400 more expensive)

Thanks for your help.

p.s. sorry about my screenname, i'm using someone else's account - i don't want you to get the idea that i think myself a "guru" when i'm only doing a science fair project

Hi,guy. Nice to meet you. I am interested in A-beta. As if A-beta 25-35 is mainly involved in mice or rats, and A-beta 1-40 or 1-42 is mainly involved in human.Maybe different A-beta have different inflammatory action on different macrophages.