simple pipetting problem - (Nov/10/2009 )
I feel kind of ridiculous posting this, but when I try pipetting Sepharose beads after washing and spinning them...well, I can't. They don't pipette up. I've cut the tips. Do I leave some of the wash buffer in there or what? I'm just using these beads initially to cut down on nonspecific binding. I don't have any problems when I need to pipette PGS beads. Suggestions? (Sorry if this seems pretty elementary..)
well i ve not done tat but may be a paste will make it more difficult to pipette. So better leave some wash buffer as little as can be pipetted out!!! Alternatively try taking it out by a pastuer pipette if the volume does not need to be accurate!!