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how to mark and identify mice/rats - (Oct/26/2009 )

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I have 50 mice for genotyping. But I don't know how to mark mice for my expirement.
What I am using is make lines on the tail with a marker, but it only lasts for 3 days.
Anyone can tell me the standard method, punch ears, cut nails?
thanks! :lol:

-fortunate-

fortunate on Oct 27 2009, 11:00 AM said:

I have 50 mice for genotyping. But I don't know how to mark mice for my expirement.
What I am using is make lines on the tail with a marker, but it only lasts for 3 days.
Anyone can tell me the standard method, punch ears, cut nails?
thanks! :)


some people punch ears... others mark them with permenant dye colors!!!
some others shave on different regions... (Tricky.. need to keep track)

-Pradeep Iyer-

some people punch ears... others mark them with permenant dye colors!!!
some others shave on different regions... (Tricky.. need to keep track)




You are helpful, but I would be grateful if you explain more or provide a link that I can refer to.

-fortunate-

try these if they help

http://www.researchtraining.org/moduletext...=606#lesson7728

http://vision.ucsd.edu/~kbranson/BransonRabaudBelongie.pdf

Best luck!!!

-Pradeep Iyer-

I use ear punching. You're going to need to be trained by someone, do you have a professor or TA who could show you? If not, one of your facility's Animal Care Technicians may be able to help you if you ask.

Here's the 'standard' way to ear punch mice, but you can use your own variation. http://www.bu.edu/research/compliance/lacu...-punching.shtml



In my experience, It's easier to number a few mice in each cage, rather than try to accurately punch every number 1-99. What I'd do is: say you have a cage with 5 mice, mark them NM (no mark, no punch), R (1 hole in right ear), L (1 hole in left ear), RR (2 in right ear), LL (2 in left ear). So your cage would look like this:

NM
R
L
RR
LL

... a cage with 2 mice in it would just be NM, R, make sense? Hope this was helpful :D I love working with my mice.

-Fungus_Dreams-

Thank you guys, that's exactly what i need!

-fortunate-

I think you should catch a mouse that have a signal with a piece of paper round its neck

-karljoesen-

You can number the mice and put them in different colour trays which will give you the account of how many mice about to go and how many you had done with.
I hope this will help.

You can learn about the more scope in this field by referring scope of biology from wiziq.

Regards
Rupam

-Rupam-

we use a permanent dye, to color the tails.
and numbering or writing valuable info. on the tray itself, works fine too.

-nightingale-

Here is an alternative method that has worked very well.

Mouse Ear Tattoo

-toxo-
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