Help needed about 5' UTR - what is 5' UTR? (Oct/17/2009 )

Hi everyone,

I am confused.
I don't know whether everything infront of ATG is considered 5'UTR or not?
for specific example,
http://www.ensembl.org/Homo_sapiens/Gene/S...ENSG00000213927

is 5'UTR CCTCCTCAGCCAGAAGGGCCTGCTCTAGGGACCCATTCCCATCCATTTCTTCTGATAAGA
CAGGCAGGCCAAGCCAGGAAGTGGGGAGGAGAAGGTGAAGGCAAGTTGGTGCTTGCCTGC
CCTCTGCTCCCACCCCACCCACAACTCCCCTGTCAGAATCTGTGTGTGCTAGTGTGAATT
TGCTTGTGATTGGATGACTGTGAAAACACAATTGGGTGGGAGAGGGACTTTGAGTATAAC
CGGGGCTATATAATTTTGTCTACGGTTGTCTGATCATGAGTATGACTTTGTGAGGTTGAC
CAAGTGATTGAGGAGTGACTTTGAATGTGTGGCTATGCGTATTTGAGTGTGAATGTCTAT
CATTAGATGCTGCTGTGGATGCTTGACTGCCCTTACTCACTGTTCCATCAGTCCCTACAT
CACACACTAATCCTTCCCCCAGCTTCACTGCCCAGAGCTTAGGAGCACATGGGAAATGGC
TATCCTAGATGAGGAAGGAGTCTGTGGCAACAGGCCGAGAATTAGGGCCCAGGAGTGTCA
CAGGTTACACAGTACATCTCCAACTGCCTCTGACTCACCTAGTGCCCCAGGTAAAAGGCA
CCCAGATAAAAGGTAGGGGAGGAGGAGAGAGAGAGAAGGAAGAGTCTAGGCTGAGCAAC ?

or just CCCAGATAAAAGGTAGGGGAGGAGGAGAGAGAGAGAAGGAAGAGTCTAGGCTGAGCAAC?

similarly, which is the 3' UTR?


any help would be appreciated.


thanks

shit, already 14 views and no answer!!

kedar on Oct 17 2009, 09:58 AM said:

shit, already 14 views and no answer!!

I dislike this kind of answer from a rude chinese. If somebody doesn't wanna help, they don't need to reply. Here we help each other, ok.

If somebody else is confused, here is the mRNA. http://www.ncbi.nlm.nih.gov/nuccore/NM_006664.2?report=fasta

My confusion is 'should all the sequence (till the very start) before the 1st ATG in the 1st exon be considered 5' UTR or just this part:
CCCAGATAAAAGGTAGGGGAGGAGGAGAGAGAGAGAAGGAAGAGTCTAGGCTGAGCAAC after which there is an inframe ATG.


thanks

zhongmindai on Oct 17 2009, 11:14 PM said:

kedar on Oct 17 2009, 09:58 AM said:

shit, already 14 views and no answer!!

I dislike this kind of question.
Its your homework, not ours.
The url you give us is not the gene's mRNA. 5'UTR (5' untranslated region) and 3'UTR is relative to mRNA, not genomic DNA sequences.

kedar on Oct 18 2009, 03:11 AM said:

I dislike this kind of answer from a rude chinese. If somebody doesn't wanna help, they don't need to reply. Here we help each other, ok.

If somebody else is confused, here is the mRNA. http://www.ncbi.nlm.nih.gov/nuccore/NM_006664.2?report=fasta

My confusion is 'should all the sequence (till the very start) before the 1st ATG in the 1st exon be considered 5' UTR or just this part:
CCCAGATAAAAGGTAGGGGAGGAGGAGAGAGAGAGAAGGAAGAGTCTAGGCTGAGCAAC after which there is an inframe ATG.


thanks

zhongmindai on Oct 17 2009, 11:14 PM said:

kedar on Oct 17 2009, 09:58 AM said:

shit, already 14 views and no answer!!

I dislike this kind of question.
Its your homework, not ours.
The url you give us is not the gene's mRNA. 5'UTR (5' untranslated region) and 3'UTR is relative to mRNA, not genomic DNA sequences.