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pulse chase experiment - (Oct/15/2009 )

hiiiii,
can someone tell me the complete protocol for pulse chase experiment??

-sssss-

sssss on Oct 15 2009, 01:17 PM said:

hiiiii,
can someone tell me the complete protocol for pulse chase experiment??


What kind of cells do you want to use and which isotope for what approach?
Lg Jana

-*Jana*-

hiiiiii,
I will transiently transfect the adherent cells and check the protein stability.i will use S-35(met) isotope.

-sssss-